Translational_Unit

Part:BBa_K4310018:Design

Designed by: Seungchan Oh   Group: iGEM22_UIncheon   (2022-10-11)


Heat shock inducible promoter + Cre recombinase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 830
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5974
    Illegal PstI site found at 830
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 226
    Illegal BglII site found at 1199
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 830
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 830
    Illegal NgoMIV site found at 2216
    Illegal NgoMIV site found at 3338
    Illegal NgoMIV site found at 3368
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1381
    Illegal BsaI site found at 2424
    Illegal BsaI site found at 3655
    Illegal BsaI site found at 6905
    Illegal BsaI.rc site found at 1660
    Illegal BsaI.rc site found at 3472
    Illegal BsaI.rc site found at 3934
    Illegal BsaI.rc site found at 4634
    Illegal BsaI.rc site found at 7184
    Illegal SapI site found at 1845
    Illegal SapI site found at 2375
    Illegal SapI site found at 4161
    Illegal SapI.rc site found at 2360
    Illegal SapI.rc site found at 2409
    Illegal SapI.rc site found at 4195


Design Notes

To make compatible to our assembly, CAMV 35S, mCherry and Tnos are amplified by overhang primers and scars of pCvHSP70A, loxP, and Cre for golden gate assembly is replaced to ours.


Source

CAMV 35S: BBa_J428074 from distribution 2022 loxP: BBa_K1680005 Cre: BBa_K4297024 mCherry: BBa_J428079 from distribution 2022 Tnos: BBa_J428082 from distribution 2022 pCvHSP70A: BBa_K4310005 eGFP: BBa_K431006 Kozak: BBa_K4310003

References